5 Questions About Autoclaving Plastic Bottles and Glass ...

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What is Autoclaving?

Autoclaving is used to sterilize plastic and glass bottles, plastic caps, medical instruments, applicators, and numerous other items that can withstand high temperatures and pressures. Products or components used in the pharmaceutical or medical field require control of microbial levels during processing and handling. Autoclaving is one of the many options used to achieve microbial limit testing on these products.

Autoclaving is used to sterilize plastic and glass bottles, plastic caps, medical instruments, applicators, and numerous other items that can withstand high temperatures and pressures. Products or components used in the pharmaceutical or medical field require control of microbial levels during processing and handling. Autoclaving is one of the many options used to achieve microbial limit testing on these products.

When used in large industrial scale, the process is also called retorts. Autoclaving has been in use for more than a hundred years, particularly in laboratory settings.

How is it done?

Autoclaving uses high-pressure stream to achieve reliable sterilization. It is the preferred method of sterilization unless the material to be sterilized can be damaged by heat or moisture.

Autoclaving uses high-pressure stream to achieve reliable sterilization. It is the preferred method of sterilization unless the material to be sterilized can be damaged by heat or moisture.

Sterilization in an autoclave is most effective when the organisms are either contacted by the steam directly or are contained in a small volume of aqueous (primarily water) liquid. Under these conditions, steam at a pressure of about 15 psi, attaining temperature of 121 C will kill all organisms and their endospores in about 15 minutes.

What plastic materials are safe for autoclaving?

Not all plastic materials are safe for autoclaving. Polypropylene (PP) and polypropylene copolymer (PPCO) containers can be autoclaved many times. Fluoropolymer products, such as Teflon PFA, FEP, or ETFE, can be autoclaved without problems. However, Polycarbonate (PC) containers can be autoclaved with caution, as they should not be exposed to alkaline detergents or steam additives, and they may withstand only up to 30 &#; 50 autoclaving cycles. Sterlizing PC reduces the mechanical strength of the material.

Not all plastic materials are safe for autoclaving. Polypropylene (PP) and polypropylene copolymer (PPCO) containers can be autoclaved many times. Fluoropolymer products, such as Teflon PFA, FEP, or ETFE, can be autoclaved without problems. However, Polycarbonate (PC) containers can be autoclaved with caution, as they should not be exposed to alkaline detergents or steam additives, and they may withstand only up to 30 &#; 50 autoclaving cycles. Sterlizing PC reduces the mechanical strength of the material.

The following resins can not be autoclaved- HDPE, LDPE, PET, PETG. Each of these material can be successfully sterilized by gas (ethylene oxide formaeldehyde).

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What living materials are safe for autoclaving?

The only recommended closure liners that can withstand autoclaving are rubber and Teflon.

The only recommended closure liners that can withstand autoclaving are rubber and Teflon.

How about Glass?

Glass is generally considered safe for autoclaving. When autoclaving glass bottles and caps together, make sure the cap is loosened prior to sending the components to autoclave to prevent the bottles from exploding. Many times an aluminum foil with autoclave tapes are placed to keep the inside of the bottle sterile. Autoclave tape will also turn black to help show the bottle has gone through the autoclaving process.

Glass is generally considered safe for autoclaving. When autoclaving glass bottles and caps together, make sure the cap is loosened prior to sending the components to autoclave to prevent the bottles from exploding. Many times an aluminum foil with autoclave tapes are placed to keep the inside of the bottle sterile. Autoclave tape will also turn black to help show the bottle has gone through the autoclaving process.

NOTE: Clean and rinse all items with distilled water before autoclaving.

Where can I get more information about Autoclaving?

While we are not an autoclaving equipment vendor, we can help answer general questions about autoclaving. Click on the button or leave a comment below.

While we are not an autoclaving equipment vendor, we can help answer general questions about autoclaving. Click on the button or leave a comment below.

So there we have it. Honestly, I&#;m hugely impressed by the print quality and performance of this homemade, recycled PET filament. Of course, working with 20 g pieces of material is a pain, but maybe someone develops an easy DIY version of something like the Palette Splice cores to easily fuse the material because all other splicing solutions I tried in the past are just horrible! Another question is the economic and ecologic feasibility of this method. Here in Germany, we have a well-working bottle deposit system where 94% of all PET bottles get recycled, simply because there is a 25 cents deposit on each bottle. 25 cents for maybe 20g of material might sound expensive, but if you do the math, that&#;s just a bit more than 12&#; per kilo for the raw material. Normal PETG filament sells for around 30&#; per kilo. And there are plenty of countries with lower or even no deposit where you could have access to free raw material. Of course, all of this is not accounting for your time, the initial invenstment and the energy used in this process. I somehow see Pul-truding a bit like stuffing your own cigarettes. It can be feasible if you have the time; if you would otherwise work a job, don&#;t even consider doing it yourself. If you leave that out of the equation, this process is way more than a gimmick and really interesting for education to demonstrate perfect recycling of otherwise discarded bottles. The most interesting application for me are actually countries and regions where people either don&#;t have the money for buying filament or it&#;s simply just not available. PET bottles are everywhere and seem to make a great source for material that&#;s strong and well usable in a wide range of applications. Of course, you need a 3D printer with an all-metal hotend and heated bed, but these have become really affordable in the last few years. What do you think? If you want to learn more, check the resources and channels linked below, especially the YouTube channel of Joshua and this crazy Russian guy named Brother, who I, unfortunately, can&#;t understand but he seems to 3D print everything and even made an extruder that directly prints with these PET bottle strips! And here&#;s the question for you. Do you think I should build myself one of these recycling machines and properly investigate use cases, feasibility, and material properties? Leave a comment down below!

JRT3D YouTube Channel: https://www.youtube.com/channel/UCsv9RMNQvMnIgBzMo57i7NA

"Brother" YouTube Channel: https://www.youtube.com/c/Brother-live

PETBot: https://bazar.preciousplastic.com/machines/extruder/extruder-fully-built/petbot/

Recreator 3D: http://recreator3d.com/

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